DNA
BS2

Part:BBa_K1039002:Design

Designed by: Aaron Bender   Group: iGEM13_Waterloo   (2013-07-08)


Bxb1 J23118 promoter switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 201
    Illegal NheI site found at 224
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 33
    Illegal BsaI.rc site found at 515


Design Notes

The transcriptional terminator J61048 was included to ensure transcription only proceeds in the expected direction. Spacer regions were added adopted from ... NheI site ...


Source

Ghosh, P., Kim, A. I. & Hatfull, G. F. (2003). The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Mol Cell 12, 1101-1111.

References